GeneTex
United States (US)

MMP15 (cleaved Tyr132) antibody

MMP15-cleaved-Tyr132-antibody-GTX86936-WB-1_18121411_562.jpg

Cat No. GTX86936

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Monkey
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:10000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

61 kDa. ( Note )

Specificity/Sensitivity

MMP15 (Cleaved-Tyr132) Antibody detects endogenous levels of fragment of activated MMP15 resulting from cleavage adjacent to Tyr132.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MMP15.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 15,Mmp-15,Mt2-Mmp,Mt2Mmp,Mtmmp2,Smcp-2,Mmp15

Background

Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.

Database

DATA IMAGES
MMP15-cleaved-Tyr132-antibody-GTX86936-WB-1_18121411_562.jpg

GTX86936 WB Image

Western blot analysis of extracts from COS7 cells, treated with etoposide 25uM 1h, using MMP15 (Cleaved-Tyr132) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339