APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse kidney , rat kidney
Predict Reactivity
Bovine, Chicken, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human MMP16. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950931
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
matrix metallopeptidase 16 , C8orf57 , MMP-X2 , MT-MMP2 , MT-MMP3 , MT3-MMP
Cellular Localization
Isoform Long: Cell membrane; Single-pass type I membrane protein; Extracellular side (Potential) , Isoform Short: Secreted , extracellular space , extracellular matrix , Cell surface
Background
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene produces at least two transcripts, one which encodes a membrane-bound form and another a soluble form of the protein. Both forms of the protein activate MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX109378 WB Image
MMP16 antibody detects MMP16 protein by Western blot analysis. A. 50 μg rat kidney lysate/extract 10 % SDS-PAGE MMP16 antibody (GTX109378) dilution: 1:1000
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GTX109378 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using MMP16(GTX109378) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109378 WB Image
MMP16 antibody detects MMP16 protein by Western blot analysis. A. 50 μg mouse kidney lysate/extract 10 % SDS-PAGE MMP16 antibody (GTX109378) dilution: 1:1000
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REFERENCE
REVIEW
MMP16 antibody
Cat. No. GTX109378
Rating
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( Average 4.5 based on 2 users reviews)
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Application
Western Blot(WB)
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( Average 4.5 based on 2 users reviews)
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Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2013.
PMID: 23472152
Date :
Anonymous submitted on 07-Nov-2011
Application Tested :
WB
Sample Species :
Rat
Sample :
Dorsal root ganglion tissue
Amount used :
30μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr