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MMP16 antibody

Anti-MMP16 antibody used in IHC (Paraffin sections) (IHC-P). GTX109378
Anti-MMP16 antibody used in Western Blot (WB). GTX109378
Anti-MMP16 antibody used in Western Blot (WB). GTX109378
Anti-MMP16 antibody used in Western Blot (WB). GTX109378

Cat No. GTX109378

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

HepG2 , mouse kidney , rat kidney , *Rat dorsal root ganglion

Predict Reactivity

Bovine, Chicken, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP16. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 16,C8Orf57,Mmp-X2,Mt-Mmp2,Mt-Mmp3,Mt3-Mmp,Mmp16

Cellular Localization

Isoform Long: Cell membrane; Single-pass type I membrane protein; Extracellular side (Potential) , Isoform Short: Secreted , extracellular space , extracellular matrix , Cell surface

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene produces at least two transcripts, one which encodes a membrane-bound form and another a soluble form of the protein. Both forms of the protein activate MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-MMP16 antibody used in IHC (Paraffin sections) (IHC-P). GTX109378

GTX109378 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using MMP16(GTX109378) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MMP16 antibody used in Western Blot (WB). GTX109378

GTX109378 WB Image

MMP16 antibody detects MMP16 protein by Western blot analysis.
A. 50 µg rat kidney lysate/extract
10 % SDS-PAGE
MMP16 antibody (GTX109378) dilution: 1:1000

Anti-MMP16 antibody used in Western Blot (WB). GTX109378

GTX109378 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX109378 diluted at 1:1000

Anti-MMP16 antibody used in Western Blot (WB). GTX109378

GTX109378 WB Image

MMP16 antibody detects MMP16 protein by Western blot analysis.
A. 50 µg mouse kidney lysate/extract
10 % SDS-PAGE
MMP16 antibody (GTX109378) dilution: 1:1000

REFERENCE
REVIEW

MMP16 antibody Cat No. GTX109378

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample Species : Rat
Sample : Dorsal root ganglion tissue
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169