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United States (US)

MMP17 antibody [N2C2], Internal

GTX105268_40142_CT_IHC_19011506_159.jpg
GTX105268_40142_IHC_19011506_959.jpg
GTX105268_40142_IFA_19011506_818.jpg
GTX105268_WB_19011506_190.jpg

Cat No. GTX105268

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1500
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MT4-MMP antibody, MMP17 antibody, MMP-17 antibody, MT4MMP antibody, matrix metalloproteinase 17 (membrane-inserted) antibody, membrane-type matrix metalloproteinase 4 antibody, membrane-type-4 matrix metalloproteinase antibody, matrix metalloproteinase-17 antibody, MTMMP4 antibody, MT-MMP 4 antibody, matrix metallopeptidase 17 (membrane-inserted) antibody

Cellular Localization

Isoform Long: Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene is considered a member of the membrane-type MMP (MT-MMP) subfamily. However, this protein is unique among the MT-MMP's in that it is a GPI-anchored protein rather than a transmembrane protein. The protein activates MMP-2 by cleavage. [provided by RefSeq]

Database

DATA IMAGES
GTX105268_40142_CT_IHC_19011506_159.jpg

GTX105268 IHC-P Image

MMP17 antibody [N2C2], Internal detects MMP17 protein at cytosol on human gastria carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastria carcinoma.
MMP17 antibody [N2C2], Internal (GTX105268) dilution: 1:1500.
Scale bar = 100 μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105268_40142_IHC_19011506_959.jpg

GTX105268 IHC-P Image

MMP17 antibody [N2C2], Internal detects MMP17 protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
MMP17 antibody [N2C2], Internal (GTX105268) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105268_40142_IFA_19011506_818.jpg

GTX105268 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MMP17(GTX105268) antibody at 1:200 dilution.

GTX105268_WB_19011506_190.jpg

GTX105268 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX105268 diluted at 1:1000

REVIEW

MMP17 antibody [N2C2], Internal Cat No. GTX105268

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 28-Nov-2013
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : gastria carcinoma tissue
Fixation : 10% formalin
Permeabilization /Antigen retrieval : CC1
Primary Antibody : 1:1500, 37°C, 1Hr
Figure Note : Scale bar = 100 μm.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169