GeneTex
United States (US)

MMP19 antibody

GTX105217_40744_20171229_WB_B_19011506_983.jpg
GTX105217_40744_CT_IHC_19011506_455.jpg

Cat No. GTX105217

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

MMP19-transfected 293T

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP19. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MMP18 antibody, RASI-1 antibody, MMP19 antibody, MMP-19 antibody, matrix metalloproteinase 18 antibody, matrix metalloproteinase RASI antibody, matrix metalloproteinase-19 antibody, MMP-18 antibody, matrix metalloproteinase 19 antibody, matrix metalloproteinase-18 antibody, matrix metallopeptidase 19 antibody

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This protein is expressed in human epidermis and it has a role in cellular proliferation as well as migration and adhesion to type I collagen. Multiple transcript variants encoding distict isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105217_40744_20171229_WB_B_19011506_983.jpg

GTX105217 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP19 antibody (GTX105217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105217_40744_CT_IHC_19011506_455.jpg

GTX105217 IHC-P Image

MMP19 antibody detects MMP19 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
MMP19 antibody (GTX105217) dilution: 1:500.
Scale bar = 100 μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REVIEW

MMP19 antibody Cat No. GTX105217

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 28-Nov-2013
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : colon carcinoma tissue
Fixation : 10% formalin
Permeabilization /Antigen retrieval : CC1
Primary Antibody : 1:500, 37°C, 1Hr
Figure Note : Scale bar = 100 μm.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf