GeneTex
United States (US)

MMP2 antibody [8B4]

GTX30147_538_IHC-P_18121410_531.jpg
GTX30147_1348_WB_18121410_324.jpg

Cat No. GTX30147

Host Mouse
Clonality Monoclonal
Clone Name 8B4
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/ml
ICC/IF 1:10 - 1:500
IHC-P 2 μg/ml
IHC-Fr 2 μg/ml
IP 1 - 2μg /100 - 500μg protein
ELISA 0.03 μg/ml
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Specificity/Sensitivity

This is specific for pro and active MMP2.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Activated recombinant human MMP2. [UniProt# P08253]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 2,Clg4,Clg4A,Mmp-2,Mmp-Ii,Mona,Tbe-1,Mmp2

Cellular Localization

Secreted,extracellular space,extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30147_538_IHC-P_18121410_531.jpg

GTX30147 IHC-P Image

IHC-P analysis of human ovary tissue using GTX30147 MMP2 antibody [8B4].
Dilution : 2.5 μg/ml

GTX30147_1348_WB_18121410_324.jpg

GTX30147 WB Image

WB analysis of pro-MMP2 (lane 1), active MMP2 (lane 2) and human pressure ulcer biopsy (lane 3) using GTX30147 MMP2 antibody [8B4].

REFERENCE