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MMP2 antibody [8B4]

Orthogonal
Anti-MMP2 antibody [8B4] used in Western Blot (WB). GTX30147
Anti-MMP2 antibody [8B4] used in IHC (Paraffin sections) (IHC-P). GTX30147

Cat. No. GTX30147

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8B4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA

Reactivity

 Paracentrotus lividus, Human, Mouse, Rat
Package
100 μl ($449)
See all MMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/ml
ICC/IF 1:10 - 1:500
IHC-P 2 μg/ml
IHC-Fr 2 μg/ml
IP 1 - 2μg /100 - 500μg protein
ELISA 0.03 μg/ml
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Product Note

This is specific for pro and active MMP2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Activated recombinant human MMP2. [UniProt# P08253]

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_369568

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 2 , CLG4 , CLG4A , MMP-2 , MMP-II , MONA , TBE-1

Cellular Localization

Membrane,Nucleus,Mitochondrion

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MMP2 antibody [8B4] used in Western Blot (WB). GTX30147

GTX30147 WB Image

WB analysis of pro-MMP2 (lane 1), active MMP2 (lane 2) and human pressure ulcer biopsy (lane 3) using GTX30147 MMP2 antibody [8B4].

Anti-MMP2 antibody [8B4] used in IHC (Paraffin sections) (IHC-P). GTX30147

GTX30147 IHC-P Image

IHC-P analysis of human ovary tissue using GTX30147 MMP2 antibody [8B4].
Dilution : 2.5 μg/ml

REFERENCE

Application Reference

PL Hsu et al. Sci Adv 2023; 9 (15) : eade3422 Targeting BRD3 eradicates nuclear TYRO3-induced colorectal cancer metastasis
Application : IHC-P
Chiarelli R et al. Int J Mol Sci 2022; 23 (22) : 0 Vanadium Modulates Proteolytic Activities and MMP-14-Like Levels during Paracentrotus lividus Embryogenesis.
Application : WB
Reactivity : Paracentrotus lividus
Jhen-Hao Jhan et al. Cancers (Basel) 2022; 14 (4) MicroRNA-375-3p Suppresses Upper Tract Urothelial Carcinoma Cell Migration and Invasion via Targeting Derlin-1.
Application : WB
Reactivity : Human
Corradi D et al. J Cardiovasc Electrophysiol 2012; 23 (3) : 271-9 Differential structural remodeling of the left-atrial posterior wall in patients affected by mitral regurgitation with or without persistent atrial fibrillation: a morphological and molecular study.
Application : IHC-P
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449