GeneTex
United States (US)

MMP2 antibody [F14 P4 D3]

GTX15772_67_ELISA_19011519_288.jpg
GTX15772_1527_WB_19011519_145.jpg

Cat No. GTX15772

Host Mouse
Clonality Monoclonal
Clone Name F14 P4 D3
Isotype IgG1
Application WB, ELISA
Reactivity Human, Rat, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200-1:500
ELISA 1-2 μg
Not tested in other applications.

Calculated MW

74 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues T(557) S L G L P P D V Q R V D(569) of human MMP-2.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 2,Clg4,Clg4A,Mmp-2,Mmp-Ii,Mona,Tbe-1,Mmp2

Cellular Localization

Cytoplasm,Extracellular matrix,Extracellular space,Membrane,Mitochondrion,Nucleus,Secreted

Background

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES
GTX15772_67_ELISA_19011519_288.jpg

GTX15772 ELISA Image

ELISA analysis of 1 µg/mL recombinant MMP2 protein diluted in carbonate/bicarbonate buffer using serial diluted GTX15772 MMP2 antibody [F14 P4 D3].

GTX15772_1527_WB_19011519_145.jpg

GTX15772 WB Image

WB analysis of 25 µg of the indicated whole cell lysates using GTX15772 MMP2 antibody [F14 P4 D3].
Dilution : 5 µg/ml