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MMP2 antibody [GT185]

Orthogonal
Anti-MMP2 antibody [GT185] used in Western Blot (WB). GTX634832

Cat. No. GTX634832

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT185

Isotype

 IgG2a

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

U87-MG , SK-N-AS

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888487

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 2 , CLG4 , CLG4A , MMP-2 , MMP-II , MONA , TBE-1

Cellular Localization

Membrane,Nucleus,Mitochondrion

Background

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-MMP2 antibody [GT185] used in Western Blot (WB). GTX634832

GTX634832 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody [GT185] (GTX634832) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Zanr? V et al. Int J Mol Sci 2024; 25 (3) Lamivudine, Doravirine, and Cabotegravir Downregulate the Expression of Human Endogenous Retroviruses (HERVs), Inhibit Cell Growth, and Reduce Invasive Capability in Melanoma Cell Lines.
Zhong X et al. Acta Pharm Sin B 2024; 14 (2) : 712-728 The lysine methyltransferase SMYD2 facilitates neointimal hyperplasia by regulating the HDAC3-SRF axis.
Cardile A et al. Antioxidants (Basel) 2023; 12 (7) Hyperforin Enhances Heme Oxygenase-1 Expression Triggering Lipid Peroxidation in BRAF-Mutated Melanoma Cells and Hampers the Expression of Pro-Metastatic Markers.
Fang ZM et al. Cell Prolif 2023; 56 (4) : e13386 Methyltransferase-like 3 suppresses phenotypic switching of vascular smooth muscle cells by activating autophagosome formation.
Application : WB
Reactivity : Human
He Y et al. Clin Epigenetics 2022; 14 (1) : 101 JIB-04, a histone demethylase Jumonji C domain inhibitor, regulates phenotypic switching of vascular smooth muscle cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations