Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody [HL1208] (GTX636525) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody [HL1208] (GTX636525) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody [HL1208] (GTX636525) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MMP2 antibody [HL1208] detects MMP2 protein at cytoplasm and nuclear envelope by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MMP2 stained by MMP2 antibody [HL1208] (GTX636525) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).