GeneTex
United States (US)

MMP2 antibody [MMP2/2C1-1D12]

GTX15773_1036_IHC-P_19011519_572.jpg

Cat No. GTX15773

Host Mouse
Clonality Monoclonal
Clone Name MMP2/2C1-1D12
Isotype IgG1
Application WB, IHC-P, IHC-Fr, IP, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
IHC-P 1:10-1:100
IHC-Fr 5 μg/ml
IP Assay dependent
ELISA 0.1 μg/ml
Not tested in other applications.

Calculated MW

74 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Activated rhMMP-2

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 2,Clg4,Clg4A,Mmp-2,Mmp-Ii,Mona,Tbe-1,Mmp2

Cellular Localization

Cytoplasm,Extracellular matrix,Extracellular space,Membrane,Mitochondrion,Nucleus,Secreted

Background

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES
GTX15773_1036_IHC-P_19011519_572.jpg

GTX15773 IHC-P Image

IHC-P analysis of human breast tissue using GTX15773 MMP2 antibody [MMP2/2C1-1D12].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20