GeneTex
United States (US)

MMP2 antibody

Orthogonal
GTX104577_40114_IFA_19011506_782.jpg
GTX104577_40800_IHC_19011506_796.jpg
GTX104577_40114_WB_M_brain_19011506_974.jpg
GTX104577_40800_WB_19011506_622.jpg
GTX104577_42599_20161027_WB_19011506_549.jpg
GTX104577_42599_20161006_WB_19011506_781.jpg
GTX104577_40800_IHC_3_19011506_351.jpg
GTX104577_42795_20170601_IHC-P_19011506_780.jpg
GTX104577_40800_IHC_2_19011506_147.jpg
GTX104577_42796_20170601_IHC-P_19011506_807.jpg
GTX104577_40800_20150327_IP_19011506_440.jpg

Cat No. GTX104577

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

PC-3 , U87-MG , SK-N-SH , U87-MG conditioned medium , mouse brain , *DU145

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 2,Clg4,Clg4A,Mmp-2,Mmp-Ii,Mona,Tbe-1,Mmp2

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104577_40114_IFA_19011506_782.jpg

GTX104577 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MMP2(GTX104577) antibody at 1:200 dilution.

GTX104577_40800_IHC_19011506_796.jpg

GTX104577 IHC-P Image

MMP2 antibody detects MMP2 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MMP2 antibody (GTX104577) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104577_40114_WB_M_brain_19011506_974.jpg

GTX104577 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX104577 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104577_40800_WB_19011506_622.jpg

GTX104577 WB Image

MMP2 antibody detects MMP2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GTX104577) diluted by 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104577_42599_20161027_WB_19011506_549.jpg

GTX104577 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GTX104577) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104577_42599_20161006_WB_19011506_781.jpg

GTX104577 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GTX104577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104577_40800_IHC_3_19011506_351.jpg

GTX104577 IHC-P Image

MMP2 antibody detects MMP2 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MMP2 antibody (GTX104577) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104577_42795_20170601_IHC-P_19011506_780.jpg

GTX104577 IHC-P Image

MMP2 antibody detects MMP2 protein at extracellular matrix in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
MMP2 antibody (GTX104577) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104577_40800_IHC_2_19011506_147.jpg

GTX104577 IHC-P Image

MMP2 antibody detects MMP2 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
MMP2 antibody (GTX104577) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104577_42796_20170601_IHC-P_19011506_807.jpg

GTX104577 IHC-P Image

MMP2 antibody detects MMP2 protein at extracellular matrix in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
MMP2 antibody (GTX104577) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104577_40800_20150327_IP_19011506_440.jpg

GTX104577 IP Image

Immunoprecipitation of MMP2 protein from U87-MG whole cell extracts using 5 μg of MMP2 antibody (GTX104577).
Western blot analysis was performed using MMP2 antibody (GTX104577).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
REVIEW

MMP2 antibody Cat No. GTX104577

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Oral cancer
Amount used : 50 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:1500, 4°C, overnightHr
Lane Description : Lane 1: OC3 with siCtrl, Lane 2: OC3 with siXXXX, Lane 3: OC3-IV2 with siCtrl, Lane 4: OC3-IV2 with siXXXX
Date : Anonymous submitted on 30-Jun-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : DU145 cells
Amount used : 55μg
Blocking : 1.5%BSA, 24°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf