U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GTX104577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GTX104577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GTX104577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MMP2 antibody detects MMP2 protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MMP2 stained by MMP2 antibody (GTX104577) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

MMP2 antibody detects MMP2 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MMP2 antibody (GTX104577) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MMP2 antibody detects MMP2 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MMP2 antibody (GTX104577) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MMP2 antibody detects MMP2 protein at extracellular matrix in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
MMP2 antibody (GTX104577) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MMP2 antibody detects MMP2 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
MMP2 antibody (GTX104577) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of MMP2 protein from U87-MG whole cell extracts using 5 μg of MMP2 antibody (GTX104577).
Western blot analysis was performed using MMP2 antibody (GTX104577).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

MMP2 antibody detects MMP2 protein at extracellular matrix in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
MMP2 antibody (GTX104577) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038

The data was published in the journal Sci Rep in 2016. PMID: 26813495