APPLICATION
Application Note
For ELISA: Use at a concentration of 0.1 - 1.0μg/ml. For IP: Use at a concentration of 3.0 - 5.0 μg/extract from 10^6 cells. For WB: Use at a concentration of 0.5 - 2μg/ml. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Product Note
This antibody is specific to MMP2, and does not cross react with MMP1, 3, 7, 8, 9, 10 or 16.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide corresponding to C-terminus of MMP2 (Human).
Purification
Immunogen affinity purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
matrix metallopeptidase 2 , CLG4 , CLG4A , MMP-2 , MMP-II , MONA , TBE-1
Cellular Localization
Membrane , Nucleus , Mitochondrion
Background
This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
Database
Research Area
DATA IMAGES
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GTX25704 WB Image
Western blot analysis of human recombinant MMP-2 with GTX25704. Protein band was detected at ~72 kDa (A); the band was blocked by the immunizing peptide (B).
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REFERENCE
REVIEW
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