APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
human MMP24-transfected 293T cells , mouse brain , SK-N-SH , IMR32
Predict Reactivity
Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human MMP24. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885740
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
matrix metallopeptidase 24 , MMP-24 , MMP25 , MT-MMP 5 , MT-MMP5 , MT5-MMP , MT5MMP , MTMMP5
Cellular Localization
Cell membrane; Single-pass type I membrane protein; Extracellular side , Processed matrix metalloproteinase-24: Secreted , extracellular space , extracellular matrix
Background
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 by cleavage. The gene has previously been referred to as MMP25 but has been renamed MMP24. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX128246 WB Image
MMP24 antibody detects MMP24 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human MMP24-transfected 293T cells
7.5 % SDS-PAGE
MMP24 antibody (GTX128246) dilution: 1:5000
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GTX128246 WB Image
MMP24 antibody detects MMP24 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5 % SDS-PAGE
MMP24 antibody (GTX128246) dilution: 1:1000
|
|
|
GTX128246 WB Image
MMP24 antibody detects MMP24 protein by western blot analysis.
A. 30 μg SK-N-SH whole cell lysate/extract
B. 30 μg IMR32 whole cell lysate/extract
7.5 % SDS-PAGE
MMP24 antibody (GTX128246) dilution: 1:1000
|
REFERENCE
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