GeneTex
United States (US)

MMP28 antibody

GTX104266_39876_IHC_19011506_729.jpg
GTX104266_39876_IFA_19011506_413.jpg
GTX104266_WB_19011506_692.jpg
GTX104266_39876_WB_R_19011506_934.jpg

Cat No. GTX104266

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP28. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EPILYSIN antibody, MM28 antibody, MMP-28 antibody, MMP25 antibody, MMP28 antibody, matrix metalloproteinase 28 antibody, matrix metalloproteinase-28, putative MMP28 gene product antibody, matrix metalloproteinase-28 antibody, matrix metallopeptidase 28 antibody

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction and tissue remodeling, and disease processes, such as asthma and metastasis. This gene encodes a secreted enzyme that degrades casein. Its expression pattern suggests that it plays a role in tissue homeostasis and in wound repair. Transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

DATA IMAGES
GTX104266_39876_IHC_19011506_729.jpg

GTX104266 IHC-P Image

MMP28 antibody detects MMP28 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma tissue.
MMP28 antibody (GTX104266) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104266_39876_IFA_19011506_413.jpg

GTX104266 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MMP28(GTX104266) antibody at 1:200 dilution.

GTX104266_WB_19011506_692.jpg

GTX104266 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX104266 diluted at 1:1000

GTX104266_39876_WB_R_19011506_934.jpg

GTX104266 WB Image

MMP28 antibody detects MMP28 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
MMP28 antibody (GTX104266) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169