GeneTex
United States (US)

MMP3 antibody [N3C1], Internal

GTX100723_IHC_19011506_378.jpg
GTX100723_WB_19011506_677.jpg
GTX100723_39988_IFA_19011506_512.jpg

Cat No. GTX100723

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HelaS3 , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CHDS6 antibody, MGC126102 antibody, MGC126103 antibody, MGC126104 antibody, MMP-3 antibody, SL-1 antibody, STMY antibody, STMY1 antibody, STR1 antibody, MMP3 antibody, matrix metalloproteinase-3 antibody, transin-1 antibody, "matrix metalloproteinase 3 (stromelysin 1, progelatinase) antibody", proteoglycanase antibody, stromelysin-1 antibody, "matrix metallopeptidase 3 (stromelysin 1, progelatinase) antibody"

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100723_IHC_19011506_378.jpg

GTX100723 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using MMP3(GTX100723) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100723_WB_19011506_677.jpg

GTX100723 WB Image

Sample(30 µg whole cell lysate)
A:Hep G2 (GTX27900)
10% SDS PAGE
GTX100723 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100723_39988_IFA_19011506_512.jpg

GTX100723 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MMP3(GTX100723) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf