GeneTex
United States (US)

MMP3 (cleaved Phe100) antibody

MMP3-cleaved-Phe100-antibody-GTX86941-WB-1_18121411_560.jpg

Cat No. GTX86941

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

43 kDa. ( Note )

Specificity/Sensitivity

MMP3 (Cleaved-Phe100) Antibody detects endogenous levels of fragment of activated MMP3 resulting from cleavage adjacent to Phe100.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MMP3.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 3,Chds6,Mmp-3,Sl-1,Stmy,Stmy1,Str1,Mmp3

Background

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Database

Research Area

DATA IMAGES
MMP3-cleaved-Phe100-antibody-GTX86941-WB-1_18121411_560.jpg

GTX86941 WB Image

Western blot analysis of extracts from A549/293/HeLa cells, treated with etoposide 25uM 24h, using MMP3 (Cleaved-Phe100) Antibody. The lane on the right is treated with the synthesized peptide.