MMP7 antibody [C2C3], C-term

Cat. No. GTX104658

Reference 16

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human, Mouse, Rat, Bovine
Package	100 μl ($399), 25 μl ($169)

See all MMP7 products

View product citations for antibody GTX104658 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Predict Reactivity

Sheep(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MMP7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241062

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 7 , MMP-7 , MPSL1 , PUMP-1

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Database

Gene ID: 4316 MMP7
UniProt: P09237 MMP7

Research Area

DATA IMAGES

GTX104658 IHC-P Image

MMP7 antibody [C2C3], C-term detects MMP7 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MMP7 stained by MMP7 antibody [C2C3], C-term (GTX104658) diluted at 1:500.
Antigen Retrieval

GTX104658 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, full-length human MMP-7 / PUMP1 protein, His Tag (4000-62.5 ng/mL). Coated protein was probed with MMP7 antibody [C2C3], C-term (GTX104658) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX104658 IHC-P Image

MMP7 antibody [C2C3], C-term detects MMP7 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
MMP7 stained by MMP7 antibody [C2C3], C-term (GTX104658) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104658 WB Image

A549 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MMP7 antibody [C2C3], C-term (GTX104658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.