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MMP7 antibody [C2C3], C-term

Anti-MMP7 antibody [C2C3], C-term used in ELISA (ELISA). GTX104658
Anti-MMP7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104658
Anti-MMP7 antibody [C2C3], C-term used in Western Blot (WB). GTX104658
Anti-MMP7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104658
Anti-MMP7 antibody [C2C3], C-term used in Western Blot (WB). GTX104658
Anti-MMP7 antibody [C2C3], C-term used in Western Blot (WB). GTX104658
Anti-MMP7 antibody [C2C3], C-term used in Western Blot (WB). GTX104658
Anti-MMP7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104658
Anti-MMP7 antibody [C2C3], C-term used in Western Blot (WB). GTX104658

Cat. No. GTX104658

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat, Bovine
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Predict Reactivity

Sheep(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MMP7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241062

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 7 , MMP-7 , MPSL1 , PUMP-1

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MMP7 antibody [C2C3], C-term used in ELISA (ELISA). GTX104658

GTX104658 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, full-length human MMP-7 / PUMP1 protein, His Tag (4000-62.5 ng/mL). Coated protein was probed with MMP7 antibody [C2C3], C-term (GTX104658) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-MMP7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104658

GTX104658 IHC-P Image

MMP7 antibody [C2C3], C-term detects MMP7 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
MMP7 stained by MMP7 antibody [C2C3], C-term (GTX104658) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MMP7 antibody [C2C3], C-term used in Western Blot (WB). GTX104658

GTX104658 WB Image

A549 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MMP7 antibody [C2C3], C-term (GTX104658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MMP7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104658

GTX104658 IHC-P Image

MMP7 antibody [C2C3], C-term detects MMP7 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MMP7 stained by MMP7 antibody [C2C3], C-term (GTX104658) diluted at 1:500.
Antigen Retrieval

Anti-MMP7 antibody [C2C3], C-term used in Western Blot (WB). GTX104658

GTX104658 WB Image

The data was published in the 2021 in Physiol Rep. PMID: 33463897
The data was published in the 2021 in Physiol Rep. PMID: 33463897

Anti-MMP7 antibody [C2C3], C-term used in Western Blot (WB). GTX104658

GTX104658 WB Image

The data was published in the journal J Cell Mol Med in 2018.PMID: 29193729
The data was published in the journal J Cell Mol Med in 2018.PMID: 29193729

Anti-MMP7 antibody [C2C3], C-term used in Western Blot (WB). GTX104658

GTX104658 WB Image

The data was published in the journal Physiol Rep in 2013. PMID: 24273653
The data was published in the journal Physiol Rep in 2013. PMID: 24273653

Anti-MMP7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104658

GTX104658 IHC-P Image

The data was published in the journal Sci Rep in 2013. PMID: 23698793
The data was published in the journal Sci Rep in 2013. PMID: 23698793

Anti-MMP7 antibody [C2C3], C-term used in Western Blot (WB). GTX104658

GTX104658 WB Image

The data was published in the journal World J Surg Oncol in 2018. PMID: 29466986
The data was published in the journal World J Surg Oncol in 2018. PMID: 29466986

REFERENCE

REVIEW

MMP7 antibody [C2C3], C-term Cat. No. GTX104658

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 4 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the 2021 in Physiol Rep. PMID: 33463897
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2018.PMID: 29193729
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Physiol Rep in 2013. PMID: 24273653
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2013. PMID: 23698793
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal World J Surg Oncol in 2018. PMID: 29466986
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169