GeneTex
United States (US)

MMP8 antibody

GTX105428_40744_IHC_19011506_483.jpg
GTX105428_40744_WB_19011506_574.jpg
GTX105428_40744_IFA_19011506_257.jpg
GTX105428_40744_IP_19011506_570.jpg

Cat No. GTX105428

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HCT116
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 8,Clg1,Hnc,Mmp-8,Pmnl-Cl,Mmp8

Cellular Localization

Cytoplasmic granule , Secreted , extracellular space , extracellular matrix (Probable)

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Database

DATA IMAGES
GTX105428_40744_IHC_19011506_483.jpg

GTX105428 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using MMP8(GTX105428) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105428_40744_WB_19011506_574.jpg

GTX105428 WB Image

Sample (30 µg of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX105428 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105428_40744_IFA_19011506_257.jpg

GTX105428 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using MMP8(GTX105428) antibody at 1:500 dilution.

GTX105428_40744_IP_19011506_570.jpg

GTX105428 IP Image

MMP8 antibody immunoprecipitates MMP8 protein in IP experiments.
IP samples: A549 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of MMP8 protein by 4 μg MMP8 antibody (GTX105428)
10 % SDS-PAGE
The immunoprecipitated MMP8 protein was detected by MMP8 antibody (GTX105428) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169