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MMP9 antibody [5G3]

Orthogonal
Anti-MMP9 antibody [5G3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60482
Anti-MMP9 antibody [5G3] used in ELISA (ELISA). GTX60482
Anti-MMP9 antibody [5G3] used in Flow cytometry (FACS). GTX60482
Anti-MMP9 antibody [5G3] used in Western Blot (WB). GTX60482
Anti-MMP9 antibody [5G3] used in Western Blot (WB). GTX60482
Anti-MMP9 antibody [5G3] used in IHC (Paraffin sections) (IHC-P). GTX60482
Anti-MMP9 antibody [5G3] used in Western Blot (WB). GTX60482

Cat. No. GTX60482

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5G3

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all MMP9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MMP9 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887974

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 9 , CLG4B , GELB , MANDP2 , MMP-9

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MMP9 antibody [5G3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60482

GTX60482 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX60482 MMP9 antibody [5G3].
Green : MMP9
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-MMP9 antibody [5G3] used in ELISA (ELISA). GTX60482

GTX60482 ELISA Image

ELISA analysis of antigen using GTX60482 MMP9 antibody [5G3].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-MMP9 antibody [5G3] used in Flow cytometry (FACS). GTX60482

GTX60482 FACS Image

FACS analysis of HeLa cells using GTX60482 MMP9 antibody [5G3].
Blue : MMP9
Red : negative control

Anti-MMP9 antibody [5G3] used in Western Blot (WB). GTX60482

GTX60482 WB Image

WB analysis of HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate using GTX60482 MMP9 antibody [5G3].

Anti-MMP9 antibody [5G3] used in Western Blot (WB). GTX60482

GTX60482 WB Image

WB analysis of human MMP9 (AA: 238-465) recombinant protein using GTX60482 MMP9 antibody [5G3].

Anti-MMP9 antibody [5G3] used in IHC (Paraffin sections) (IHC-P). GTX60482

GTX60482 IHC-P Image

IHC-P analysis of human brain tissue using GTX60482 MMP9 antibody [5G3].

Anti-MMP9 antibody [5G3] used in Western Blot (WB). GTX60482

GTX60482 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2017. PMID: 29296214

REFERENCE

Application Reference

Mudatsir et al. Ann Med Surg (Lond) 2023; 85 (9) : 4277-4282 Relationship between metalloproteinase-9 (MMP-9) expression and clinicopathology in colorectal cancer: a cross-sectional study.
Sun K et al. Oncotarget 2017; 8 (64) : 107932-107946 NCOA5 promotes proliferation, migration and invasion of colorectal cancer cells via activation of PI3K/AKT pathway.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399