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MMP9 antibody [N2C1], Internal

Orthogonal
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in Immunohistochemistry (IHC). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458

Cat. No. GTX100458

Reference 61
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μl ($399),
25 μl ($169)
See all MMP9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Predict Reactivity

Rabbit, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950936

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 9 , CLG4B , GELB , MANDP2 , MMP-9

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458

GTX100458 WB Image

Orthogonal

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP9 antibody [N2C1], Internal (GTX100458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458

GTX100458 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP9 antibody [N2C1], Internal (GTX100458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458

GTX100458 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP9 antibody [N2C1], Internal (GTX100458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MMP9 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100458

GTX100458 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MMP9(GTX100458) antibody at 1:200 dilution.

Anti-MMP9 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100458

GTX100458 IHC-P Image

MMP9 antibody [N2C1], Internal detects MMP9 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
MMP9 stained by MMP9 antibody [N2C1], Internal (GTX100458) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458

GTX100458 WB Image

Orthogonal

Untreated (–) and treated (+) U2OS whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP9 antibody [N2C1], Internal (GTX100458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MMP9 antibody [N2C1], Internal used in Immunohistochemistry (IHC). GTX100458

GTX100458 IHC Image

Orthogonal

The data was published in the journal Antioxidants (Basel) in 2020.PMID: 32218152

Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458

GTX100458 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2017.PMID: 28489070

Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458

GTX100458 WB Image

Orthogonal

The data was published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038

Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458

GTX100458 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 26813495

REFERENCE

Application Reference

Zhong X et al. Acta Pharm Sin B 2024; 14 (2) : 712-728 The lysine methyltransferase SMYD2 facilitates neointimal hyperplasia by regulating the HDAC3-SRF axis.
Wu WS et al. Am J Cancer Res 2023; 13 (10) : 4903-4917 Preclinical trial of targeting the Hic-5-mediated pathway to prevent the progression of hepatocellular carcinoma.
Kim YH et al. Nat Commun 2023; 14 (1) : 7619 Mid-old cells are a potential target for anti-aging interventions in the elderly.
Fang ZM et al. Cell Prolif 2023; 56 (4) : e13386 Methyltransferase-like 3 suppresses phenotypic switching of vascular smooth muscle cells by activating autophagosome formation.
Application : WB
Reactivity : Human
Chang TM et al. Int J Biol Sci 2023; 19 (5) : 1455-1470 CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

MMP9 antibody [N2C1], Internal Cat. No. GTX100458

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : SCC4 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM ADC treatment for 24h.
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: treated (TSP-2; 3g/mL) MG63 whole cell lysate, Lane 3: treated (TSP-2; 10g/mL) MG63 whole cell lysate
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Date : Anonymous submitted on 05-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 70μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Zhong X et al. Acta Pharm Sin B 2024; 14 (2):712-728 The lysine methyltransferase SMYD2 facilitates neointimal hyperplasia by regulating the HDAC3-SRF axis.
Wu WS et al. Am J Cancer Res 2023; 13 (10):4903-4917 Preclinical trial of targeting the Hic-5-mediated pathway to prevent the progression of hepatocellular carcinoma.
Kim YH et al. Nat Commun 2023; 14 (1):7619 Mid-old cells are a potential target for anti-aging interventions in the elderly.
Fang ZM et al. Cell Prolif 2023; 56 (4):e13386 Methyltransferase-like 3 suppresses phenotypic switching of vascular smooth muscle cells by activating autophagosome formation.
Application : WB
Reactivity : Human
Chang TM et al. Int J Biol Sci 2023; 19 (5):1455-1470 CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway.
Application : WB
Reactivity : Human
Lin YW et al. Cells 2023; 12 (3) Proteoglycan SPOCK1 as a Poor Prognostic Marker Promotes Malignant Progression of Clear Cell Renal Cell Carcinoma via Triggering the Snail/Slug-MMP-2 Axis-Mediated Epithelial-to-Mesenchymal Transition.
Application : WB
Reactivity : Human
Bao X et al. Invest Ophthalmol Vis Sci 2023; 64 (3):14 T-Cell Repertoire Analysis in the Conjunctiva of Murine Dry Eye Model.
Application : IHC-P
Reactivity : Mouse
Wang Y et al. BMC Mol Cell Biol 2022; 23 (1):49
Application : WB
Reactivity : Human
Xie T et al. Cardiovasc Ther 2022; 2022:7576388 Heme Oxygenase 1/Peroxisome Proliferator-Activated Receptor Gamma Pathway Protects Intimal Hyperplasia and Mitigates Arteriovenous Fistula Dysfunction by Regulating Oxidative Stress and Inflammatory Response.
Application : WB
Reactivity : Mouse
He Y et al. Clin Epigenetics 2022; 14 (1):101 JIB-04, a histone demethylase Jumonji C domain inhibitor, regulates phenotypic switching of vascular smooth muscle cells.
Application : WB
Reactivity : Human
Xuehui Fan et al. Front Pharmacol 2022; 13:834948 S1PR3, as a Core Protein Related to Ischemic Stroke, is Involved in the Regulation of Blood-Brain Barrier Damage.
Reactivity : Mouse
Meng-Hui Zhang et al. Bioengineered 2022; 13 (4):10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.
Application : WB
Hsiao-Ya Tsai et al. Mol Ther Nucleic Acids 2022; 27:656-669 miR-424/322 protects against abdominal aortic aneurysm formation by modulating the Smad2/3/runt-related transcription factor 2 axis.
Application : WB
Reactivity : Human
Yang C et al. Oncol Lett 2022; 23 (4):139 NRBP1 negatively regulates SALL4 to reduce the invasion and migration, promote apoptosis and increase the sensitivity to chemotherapy drugs of breast cancer cells.
Application : WB
Reactivity : Human
Tsai HY et al. Cell Press 2022; 27:656-669 miR-424/322 protects against abdominal aortic aneurysm formation by modulating the Smad2/3/runt-related transcription factor 2 axis.
Application : WB
Reactivity : Human
Xin Yi et al. Front Cardiovasc Med 2021; 8:692856 The Expression Patterns and Roles of Lysyl Oxidases in Aortic Dissection.
Application : WB
Reactivity : Human
Xu C et al. Oxid Med Cell Longev 2021; 2021:2502324 Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm.
Application : WB, IHC-P
Reactivity : Mouse
Coffey EC et al. J Dev Biol 2021; 9 (4) Lysosomal Function Impacts the Skeletal Muscle Extracellular Matrix.
Wu TT et al. Antioxidants (Basel) 2021; 10 (7) 3H-1,2-Dithiole-3-Thione Protects Lens Epithelial Cells against Fructose-Induced Epithelial-Mesenchymal Transition via Activation of AMPK to Eliminate AKR1B1-Induced Oxidative Stress in Diabetes Mellitus.
Application : WB
Reactivity : Rat
Harn HI et al. Nat Commun 2021; 12 (1):2595 Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.
Application : IHC-P, IHC-Wm
Reactivity : Mouse
Li X et al. J Cell Mol Med 2021; 25 (4):1939-1948 GDF-5 induces epidermal stem cell migration via RhoA-MMP9 signalling.
Application : WB, ICC/IF
Reactivity : Mouse
Murata K et al. J Nat Med 2021; 75 (1):142-155 Oral administration of Jumihaidokuto inhibits UVB-induced skin damage and prostaglandin E2 production in HR-1 hairless mice.
Application : IHC-Fr
Reactivity : Mouse
Chi-Wen Luo et al. Am J Cancer Res 2020; 10 (12):4325-4341 The CDK6-c-Jun-Sp1-MMP-2 axis as a biomarker and therapeutic target for triple-negative breast cancer.
Application : WB, IHC-P
Reactivity : Human, Mouse
Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Li M et al. Cancer Manag Res 2020; 12:9159-9171
Application : WB
Reactivity : Human
Tsai MH et al. Cells 2020; 9 (8) Ischemic Stroke Risk Associated with Mitochondrial Haplogroup F in the Asian Population.
Application : WB
Reactivity : Human
Liu Y et al. Elife 2020; 9 Arginine methylation of SHANK2 by PRMT7 promotes human breast cancer metastasis through activating endosomal FAK signalling.
Application : WB
Reactivity : Human
Huang CW et al. FASEB J 2020; 34 (7):9678-9693 Irisin, an exercise myokine, potently suppresses tumor proliferation, invasion, and growth in glioma.
Application : WB
Reactivity : Human
Xiao L et al. Life Sci 2020; 253:117740 Roles of N-terminal Annexin A2 phosphorylation sites and miR-206 in colonic adenocarcinoma.
Application : WB
Reactivity : Human
Wu TT et al. Antioxidants (Basel) 2020; 9 (4) AKR1B1-Induced Epithelial-Mesenchymal Transition Mediated by RAGE-Oxidative Stress in Diabetic Cataract Lens.
Reactivity : Human
Chung TW et al. Onco Targets Ther 2019; 12:6119-6131 Antitumor effect of kurarinone and underlying mechanism in small cell lung carcinoma cells.
Application : WB
Reactivity : Human
Fan YY et al. Int Immunopharmacol 2019; 77:105911 Obeticholic acid prevents carbon tetrachloride-induced liver fibrosis through interaction between farnesoid X receptor and Smad3.
Application : WB
Reactivity : Mouse
Yingqi Liu et al. bioRxiv 2019; (Epub) The PRMT7-dependent methylation of shank2 modulates invasion-proliferation switching during breast cancer metastasis.
Liu WL et al. Molecules 2019; 22 (6):pii: E929 Curcumin Inhibits LIN-28A through the Activation of miRNA-98 in the Lung Cancer Cell Line A549.
Application : WB
Reactivity : Human
Yu CY et al. Biochem Biophys Res Commun 2018; 506 (4):901-906 Transforming growth factor alpha promotes tumorigenesis and regulates epithelial-mesenchymal transition modulation in colon cancer.
Application : WB
Reactivity : Human
Tsai TH et al. Int J Mol Sci 2018; 19 (10) Deletion of RasGRF1 Attenuated Interstitial Fibrosis in Streptozotocin-Induced Diabetic Cardiomyopathy in Mice through Affecting Inflammation and Oxidative Stress.
Application : WB
Reactivity : Mouse
Fukushima R et al. J Cancer 2018; 9 (18):3326-3333 Overexpression of Translocation Associated Membrane Protein 2 Leading to Cancer-Associated Matrix Metalloproteinase Activation as a Putative Metastatic Factor for Human Oral Cancer.
Application : WB
Reactivity : Human
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
Lopez-Charcas O et al. Sci Rep 2018; 8 (1):12995 The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity.
Application : WB
Reactivity : Human
Lee H et al. Exp Mol Med 2018; 50 (1):e430 Proangiogenic functions of an RGD-SLAY-containing osteopontin icosamer peptide in HUVECs and in the postischemic brain.
Application : WB
Reactivity : Human
Amador C. V. et al. J Clin Exp Pathol 2017; 7:6 MUC1 and C-Met Affects Proliferation, Intercellular Junctions and Invasion in Two Head and Neck Carcinoma Cell Lines.
Application : WB, ICC/IF
Reactivity : Human
Kim YH et al. Nat Commun 2017; 8:15208 Senescent tumor cells lead the collective invasion in thyroid cancer.
Jiang C et al. Exp Cell Res 2017; (Epub) Treponema pallidum flagellins stimulate MMP-9 and MMP-13 expression via TLR5 and MAPK/NF-κB signaling pathways in human epidermal keratinocytes.
Application : WB
Reactivity : Human
Hsu JY et al. FASEB J 2017; (Epub) Epidermal growth factor-induced pyruvate dehydrogenase kinase 1 expression enhances head and neck squamous cell carcinoma metastasis via up-regulation of fibronectin.
Application : WB
Reactivity : Human
Chao W et al. Molecules 2017; 22 (4) 3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.
Application : WB
Reactivity : Human
Cai HK et al. Onco Targets Ther 2017; 10:1269-1278 MicroRNA-194 modulates epithelial-mesenchymal transition in human colorectal cancer metastasis.
Application : WB
Reactivity : Human
Gao Y et al. Sci Rep 2016; 6:19874 The dual function of PRMT1 in modulating epithelial-mesenchymal transition and cellular senescence in breast cancer cells through regulation of ZEB1.
Application : WB
Reactivity : Human
Barajas-Castaneda LM et al. Journal of Immunology Research 2016; 2016 (Article ID 8519648):10 Overexpression of MMP-3 and uPA with Diminished PAI-1 Related to Metastasis in Ductal Breast Cancer Patients Attending a Public Hospital in Mexico City.
Application : IHC-P
Reactivity : Human
Lee CY et al. Oncol Rep 2016; (Epub) Inhibition of human colorectal cancer metastasis by notoginsenoside R1, an important compound from Panax notoginseng.
Wang CY et al. J Cell Physiol 2016; (Epub) Primary Cilium-Regulated EG-VEGF Signaling Facilitates Trophoblast Invasion.
Application : WB
Reactivity : Human
Nakamura K et al. Mol Cancer Res 2016; (Epub) Exosomes Promote Ovarian Cancer Cell Invasion through Transfer of CD44 to Peritoneal Mesothelial Cells.
Su MT et al. Biofactors 2016; miR-346 and miR-582-3p-regulated EG-VEGF expression and trophoblast invasion via matrix metalloproteinases 2 and 9.
Application : WB
Reactivity : Human
Hsieh SL et al. Am J Chin Med 2016; 44 (4):851-70 Effects of Panax notoginseng on the Metastasis of Human Colorectal Cancer Cells.
Chen WL et al. PLoS One 2015; 10 (9):e0137979 Mycobacterium tuberculosis Upregulates TNF-α Expression via TLR2/ERK Signaling and Induces MMP-1 and MMP-9 Production in Human Pleural Mesothelial Cells.
Application : WB
Reactivity : Human
Yeh CY et al. Infect Immun 2015; 83 (6):110639 Activated human valvular interstitial cells sustain interleukin-17 production to recruit neutrophils in infective endocarditis.
Ho WT et al. Am J Pathol 2015; 185 (8):2158-67 Inhibition of matrix metalloproteinase activity reverses corneal endothelial-mesenchymal transition.
Application : WB
Reactivity : Bovine
Tsai CH et al. Biochim Biophys Acta 2015; 1852 (5):851-61 Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA.
Application : WB
Reactivity : Human
Wai-Theng Lee et al. Food and Chemical Toxicology 2015; 78:33-41 Antroquinonol from Antrodia Camphorata suppresses breast tumor migration/invasion through inhibiting ERK-AP-1- and AKT-NF-κBdependent MMP-9 and epithelial-mesenchymal transition expressions
Application : WB
Reactivity : Human
Ruiz-Morales JM et al. Tumour Biol 2014; Neutrophil gelatinase-associated lipocalin (NGAL) and matrix metalloproteinase-9 (MMP-9) prognostic value in lung adenocarcinoma.
Application : IHC-P
Reactivity : Human
Yao R et al. Cancer Res 2014; PRMT7 Induces Epithelial-to-Mesenchymal Transition and Promotes Metastasis in Breast Cancer.
Application : WB
Reactivity : Human
Hsu HY et al. Evid Based Complement Alternat Med 2012; 2012:819632 Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169