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MMP9 antibody [N2C1], Internal

Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458
Anti-MMP9 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100458

Cat. No. GTX100458

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

Human, Mouse, Rat, Bovine
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Predict Reactivity

Rabbit, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950936

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 9 , CLG4B , GELB , MANDP2 , MMP-9

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458

GTX100458 WB Image

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP9 antibody [N2C1], Internal (GTX100458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MMP9 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100458

GTX100458 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MMP9(GTX100458) antibody at 1:200 dilution.

Anti-MMP9 antibody [N2C1], Internal used in Western Blot (WB). GTX100458

GTX100458 WB Image

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP9 antibody [N2C1], Internal (GTX100458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MMP9 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100458

GTX100458 IHC-P Image

MMP9 antibody [N2C1], Internal detects MMP9 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
MMP9 stained by MMP9 antibody [N2C1], Internal (GTX100458) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

MMP9 antibody [N2C1], Internal Cat. No. GTX100458

Rating ( Average 5 based on 7 users reviews)
Application
Western Blot(WB) ( Average 5 based on 6 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 28489070
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Antioxidants (Basel) in 2020.PMID: 32218152
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : SCC4 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM ADC treatment for 24h.
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 26813495
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: treated (TSP-2; 3g/mL) MG63 whole cell lysate, Lane 3: treated (TSP-2; 10g/mL) MG63 whole cell lysate
Date : Anonymous submitted on 05-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 70μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
Wang Y et al. BMC Mol Cell Biol 2022; 23 (1):49
Application : WB
Reactivity : Human
Li M et al. Cancer Manag Res 2020; 12:9159-9171
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production