GeneTex
United States (US)

MMP9 antibody

Anti-MMP9 antibody used in Western Blot (WB). GTX31891
Anti-MMP9 antibody used in IHC (Paraffin sections) (IHC-P). GTX31891
Anti-MMP9 antibody used in IHC (Paraffin sections) (IHC-P). GTX31891

Cat No. GTX31891

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

Mouse Lung Tissue Lysate(GTX27933) , Human Lung Tissue Slide

Specificity/Sensitivity

At least three isoforms of MMP9 are known to exist; this antibody only recognizes the two longest isoforms.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MMP9 antibody was raised against a 16 amino acid peptide near the center of human MMP9.The immunogen is located within amino acids 260 - 310 of MMP9 .

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 9,Clg4B,Gelb,Mandp2,Mmp-9,Mmp9

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-MMP9 antibody used in Western Blot (WB). GTX31891

GTX31891 WB Image

WB analysis of mouse lung tissue lysate using GTX31891 MMP9 antibody.
Working concentration : 1 μg/ml

Anti-MMP9 antibody used in IHC (Paraffin sections) (IHC-P). GTX31891

GTX31891 IHC-P Image

IHC-P analysis of human lung tissue using GTX31891 MMP9 antibody.
Working concentration : 2.5 μg/ml

Anti-MMP9 antibody used in IHC (Paraffin sections) (IHC-P). GTX31891

GTX31891 IHC-P Image

IHC-P analysis of human lung tissue using GTX31891 MMP9 antibody.
Working concentration : 20 μg/ml