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MNT antibody [HL2309]

Recombinant antibody Orthogonal
Anti-MNT antibody [HL2309] used in Western Blot (WB). GTX638472
Anti-MNT antibody [HL2309] used in IHC (Paraffin sections) (IHC-P). GTX638472
Anti-MNT antibody [HL2309] used in Western Blot (WB). GTX638472
Anti-MNT antibody [HL2309] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638472

Cat. No. GTX638472

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2309

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MNT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human MNT.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAX network transcriptional repressor , MAD6 , MXD6 , ROX , bHLHd3

Cellular Localization

Nucleus

Background

The Myc/Max/Mad network comprises a group of transcription factors that co-interact to regulate gene-specific transcriptional activation or repression. This gene encodes a protein member of the Myc/Max/Mad network. This protein has a basic-Helix-Loop-Helix-zipper domain (bHLHzip) with which it binds the canonical DNA sequence CANNTG, known as the E box, following heterodimerization with Max proteins. This protein is likely a transcriptional repressor and an antagonist of Myc-dependent transcriptional activation and cell growth. This protein represses transcription by binding to DNA binding proteins at its N-terminal Sin3-interaction domain. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MNT antibody [HL2309] used in Western Blot (WB). GTX638472

GTX638472 WB Image

Orthogonal

A431 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MNT antibody [HL2309] (GTX638472) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MNT antibody [HL2309] used in IHC (Paraffin sections) (IHC-P). GTX638472

GTX638472 IHC-P Image

MNT antibody [HL2309] detects MNT protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
MNT stained by MNT antibody [HL2309] (GTX638472) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MNT antibody [HL2309] used in Western Blot (WB). GTX638472

GTX638472 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MNT antibody [HL2309] (GTX638472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MNT antibody [HL2309] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638472

GTX638472 ICC/IF Image

MNT antibody [HL2309] detects MNT protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MNT stained by MNT antibody [HL2309] (GTX638472) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations