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United States (US)

MOG antibody [C2C3], C-term

GTX106283_39701_20170705_IFA_R_19011506_183.jpg
GTX106283_39701_20170807_IHC-Fr_M_19011506_834.jpg
GTX106283_39701_20171006_WB_M_R_19011506_609.jpg

Cat No. GTX106283

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Mouse, Rat
See all MOG products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MOG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myelin Oligodendrocyte Glycoprotein,Btn6,Btnl11,Mogig2,Nrclp7,Mog

Cellular Localization

Isoform 1: Cell membrane; Multi-pass membrane protein , Isoform 5: Cell membrane; Multi-pass membrane protein , Isoform 2: Cell membrane; Single-pass type I membrane protein , Isoform 3: Cell membrane; Single-pass type I membrane protein , Isoform 4: Cell

Background

The product of this gene is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. Due to this localization, it is a primary target antigen involved in immune-mediated demyelination. This protein may be involved in completion and maintenance of the myelin sheath and in cell-cell communication. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106283_39701_20170705_IFA_R_19011506_183.jpg

GTX106283 ICC/IF Image

MOG antibody [C2C3], C-term detects MOG protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MOG protein stained by MOG antibody [C2C3], C-term (GTX106283) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GTX106283_39701_20170807_IHC-Fr_M_19011506_834.jpg

GTX106283 IHC-Fr Image

MOG antibody [C2C3], C-term detects MOG protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MOG protein stained by MOG antibody [C2C3], C-term (GTX106283) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GTX106283_39701_20171006_WB_M_R_19011506_609.jpg

GTX106283 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MOG antibody [C2C3], C-term (GTX106283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE

Application Reference

Inamura N et al. Neurobiol Dis. 2018; 120 : 51-62 Developmental defects and aberrant accumulation of endogenous psychosine in oligodendrocytes in a murine model of Krabbe disease.
Application : WB
Reactivity : Mouse
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169