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MOG antibody [C2C3], C-term

Anti-MOG antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106283
Anti-MOG antibody [C2C3], C-term used in Western Blot (WB). GTX106283
Anti-MOG antibody [C2C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX106283

Cat. No. GTX106283

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MOG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MOG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241065

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myelin oligodendrocyte glycoprotein , BTN6 , BTNL11 , MOGIG2 , NRCLP7

Cellular Localization

Isoform 1: Cell membrane; Multi-pass membrane protein , Isoform 5: Cell membrane; Multi-pass membrane protein , Isoform 2: Cell membrane; Single-pass type I membrane protein , Isoform 3: Cell membrane; Single-pass type I membrane protein , Isoform 4: Cell

Background

The product of this gene is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. Due to this localization, it is a primary target antigen involved in immune-mediated demyelination. This protein may be involved in completion and maintenance of the myelin sheath and in cell-cell communication. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MOG antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106283

GTX106283 ICC/IF Image

MOG antibody [C2C3], C-term detects MOG protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MOG protein stained by MOG antibody [C2C3], C-term (GTX106283) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-MOG antibody [C2C3], C-term used in Western Blot (WB). GTX106283

GTX106283 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MOG antibody [C2C3], C-term (GTX106283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MOG antibody [C2C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX106283

GTX106283 IHC-Fr Image

MOG antibody [C2C3], C-term detects MOG protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MOG protein stained by MOG antibody [C2C3], C-term (GTX106283) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Inamura N et al. Neurobiol Dis. 2018; 120 : 51-62 Developmental defects and aberrant accumulation of endogenous psychosine in oligodendrocytes in a murine model of Krabbe disease.
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169