GeneTex
United States (US)

MOLT-4 whole cell lysate

Cat No. GTX27912

Application WB
Species Human
PRODUCT

Product Description

Molt4 lysate was prepared by homogenization in modified RIPA buffer. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The Jurkat lysate was boiled for 5 min in 1 x SDS sample buffer containing 5% β-mercaptoethanol.
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

1 x SDS sample buffer (50 mM Tris-HCl (pH 6.8), 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2mg/ml

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Package List Price ($)
$ 199