APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , Rat2 , Neuro2A , Raw264.7 , C2C12
Predict Reactivity
Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.88 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human MP1 Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950858
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 , MAP2K1IP1 , MAPBP , MAPKSP1 , MP1 , PRO0633 , Ragulator3
Background
This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it regulates late endosomal traffic and cell proliferation. Multiple transcript variants are expressed by this gene, but only one variant is thought to express a protein. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX111032 WB Image
MP1 antibody detects MP1 protein by Western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg Raw264.7 whole cell lysate/extract C. 30 μg C2C12 whole cell lysate/extract 15 % SDS-PAGE MP1 antibody (GTX111032) dilution: 1:1000
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GTX111032 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MP1(GTX111032) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111032 WB Image
Sample (30 ug of whole cell lysate) A: 293T 15% SDS PAGE GTX111032 diluted at 1:1000
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GTX111032 WB Image
MP1 antibody detects MP1 protein by Western blot analysis. A. 30 μg Rat2 whole cell lysate/extract 15 % SDS-PAGE MP1 antibody (GTX111032) dilution: 1:1000
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REFERENCE
There are currently no references for MP1 antibody [N1C3] (GTX111032). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MP1 antibody [N1C3] (GTX111032). Be the first to share your experience with this product.