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MPI antibody

KO/KD
Anti-MPI antibody used in Western Blot (WB). GTX103682
Anti-MPI antibody used in IHC (Paraffin sections) (IHC-P). GTX103682
Anti-MPI antibody used in Western Blot (WB). GTX103682
Anti-MPI antibody used in Western Blot (WB). GTX103682

Cat. No. GTX103682

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all MPI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , PC-12 , Rat2 , HeLa

Predict Reactivity

Mouse, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MPI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950938

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mannose phosphate isomerase , CDG1B , PMI , PMI1

Cellular Localization

Cytoplasm

Background

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MPI antibody used in Western Blot (WB). GTX103682

GTX103682 WB Image

KO/KD

Wild-type (WT) and MPI knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MPI antibody (GTX103682) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MPI antibody used in IHC (Paraffin sections) (IHC-P). GTX103682

GTX103682 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Gastric CA N87 xenograft, using MPI(GTX103682) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MPI antibody used in Western Blot (WB). GTX103682

GTX103682 WB Image

MPI antibody detects MPI protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
MPI antibody (GTX103682) dilution: 1:1000

Anti-MPI antibody used in Western Blot (WB). GTX103682

GTX103682 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX103682 diluted at 1:1000

REFERENCE

Application Reference

Hern?ndez-Res?ndiz R et al. Cureus 2024; 16 (1) : e51765 Calcified Pilocytic Astrocytomas and Calcifying Pseudoneoplasms of the Neuraxis: A Diagnostic Challenge.
Kleinehr J et al. PLoS Pathog 2023; 19 (7) : e1010986 Glycolytic interference blocks influenza A virus propagation by impairing viral polymerase-driven synthesis of genomic vRNA.
Harada Y et al. Elife 2023; 12 Metabolic clogging of mannose triggers dNTP loss and genomic instability in human cancer cells.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169