GeneTex
  • Country / Location Selection

United States (US)

MPI antibody

Anti-MPI antibody used in Western Blot (WB). GTX103682
Anti-MPI antibody used in Western Blot (WB). GTX103682
Anti-MPI antibody used in Western Blot (WB). GTX103682
Anti-MPI antibody used in IHC (Paraffin sections) (IHC-P). GTX103682

Cat No. GTX103682

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , PC-12 , Rat2

Predict Reactivity

Mouse, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MPI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

mannose phosphate isomerase , CDG1B , PMI , PMI1

Cellular Localization

Cytoplasm

Background

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. [provided by RefSeq]

Database

DATA IMAGES
Anti-MPI antibody used in Western Blot (WB). GTX103682

GTX103682 WB Image

Wild-type (WT) and MPI knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MPI antibody (GTX103682) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MPI antibody used in Western Blot (WB). GTX103682

GTX103682 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX103682 diluted at 1:1000

Anti-MPI antibody used in Western Blot (WB). GTX103682

GTX103682 WB Image

MPI antibody detects MPI protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
MPI antibody (GTX103682) dilution: 1:1000

Anti-MPI antibody used in IHC (Paraffin sections) (IHC-P). GTX103682

GTX103682 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Gastric CA N87 xenograft, using MPI(GTX103682) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for MPI antibody (GTX103682). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MPI antibody (GTX103682). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169