GeneTex
United States (US)

MPRIP antibody

MPRIP-antibody-GTX87982-IHC-1_18121411_542.jpg

Cat No. GTX87982

Host Rabbit
Clonality Polyclonal
Application IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

IHC: 1:50~1:100
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

116 kDa. ( Note )

Specificity/Sensitivity

MPRIP Antibody detects endogenous levels of total MPRIP protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MPRIP.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myosin Phosphatase Rho Interacting Protein,M-Rip,Mrip,Rhoip3,Rip3,P116Rip,Mprip

Cellular Localization

Colocalizes with F-actin.

Background

Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.

Database

DATA IMAGES
MPRIP-antibody-GTX87982-IHC-1_18121411_542.jpg

GTX87982 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using MPRIP Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339