Wild-type (WT) and MPST knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MPST antibody [C2C3], C-term (GTX108274) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MPST antibody [C2C3], C-term (GTX108274) diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using MPST(GTX108274) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MPST antibody [C2C3], C-term (GTX108274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

The data was published in the 2022 in Biomolecules. PMID: 35204649