GeneTex
United States (US)

MPST antibody [C2C3], C-term

GTX108274_39778_20160421_WB_M_liver_19011507_518.jpg
GTX108274_39778_20190215_WB_KO_watermark_19030801_876.jpg
GTX108274_WB_19011507_955.jpg
GTX108274_39778_IHC_19011507_804.jpg

Cat No. GTX108274

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , mouse liver
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MPST. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mercaptopyruvate Sulfurtransferase,Mst,Tst2,Tum1,Mpst

Cellular Localization

Cytoplasm

Background

This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108274_39778_20160421_WB_M_liver_19011507_518.jpg

GTX108274 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MPST antibody [C2C3], C-term (GTX108274) diluted at 1:500.

GTX108274_39778_20190215_WB_KO_watermark_19030801_876.jpg

GTX108274 WB Image

Wild-type (WT) and MPST knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MPST antibody [C2C3], C-term (GTX108274) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX108274_WB_19011507_955.jpg

GTX108274 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX108274 diluted at 1:500

GTX108274_39778_IHC_19011507_804.jpg

GTX108274 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using MPST(GTX108274) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169