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STING antibody

KO/KD
Anti-STING antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85266
Anti-STING antibody used in Western Blot (WB). GTX85266
Anti-STING antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85266

Cat. No. GTX85266

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all MPYS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

A-20 Cell Slide(GTX25541) , A20 Cell Lysate(GTX27180) , Jurkat Cell Lysate(GTX27899) , K562 Cell Lysate(GTX27911)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MPYS antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human MPYS.The immunogen is located within amino acids 260 - 310 of MPYS.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

Knockdown/Knockout validation was supported by references (PMID: 35538059).

TARGET

Synonyms

transmembrane protein 173 , ERIS , MITA , MPYS , NET23 , SAVI , STING , STING-beta , hMITA , hSTING

Cellular Localization

Endoplasmic reticulum membrane,Mitochondrion outer membrane,Cell membrane,Cytoplasm

Background

MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.

Database

Research Area

DATA IMAGES

Anti-STING antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85266

GTX85266 ICC/IF Image

ICC/IF analysis of A-20 cells using GTX85266 STING antibody.
Working concentration : 5 μg/ml

Anti-STING antibody used in Western Blot (WB). GTX85266

GTX85266 WB Image

WB analysis of (A) K562 and (B) Jurkat cell lysate using GTX85266 STING antibody.
Working concentration : 1 μg/ml

Anti-STING antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85266

GTX85266 ICC/IF Image

ICC/IF analysis of A-20 cells using GTX85266 STING antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Meiling Yan et al. Cell Death Discov 2022; 8 (1) : 258 Mitochondrial damage and activation of the cytosolic DNA sensor cGAS-STING pathway lead to cardiac pyroptosis and hypertrophy in diabetic cardiomyopathy mice.
Application : WB, ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399