MRCK alpha antibody, N-term

Cat. No. GTX89739

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	IHC-P
Reactivity	Human
Package	100 μg ($399)

See all MRCK alpha products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
IHC-P	3-6μg/ml
Note : IHC-P Human Cerebellum shows cytoplasm staining in purkinje cells.

Not tested in other applications.

Calculated MW

197 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89739-PEP).

Product Note

This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence SGEVRLRQLEQFI-C, from the N Terminus of the protein sequence according to NP_055641.3; NP_003598.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDC42 binding protein kinase alpha , MRCK , MRCKA , PK428

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described. [provided by RefSeq, Sep 2018]

Database

Gene ID: 8476 CDC42BPA
UniProt: Q5VT25 CDC42BPA

Research Area

DATA IMAGES

GTX89739 IHC-P Image

IHC-P analysis of human cerebellum using GTX89739 MRCK alpha antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty