GeneTex
United States (US)

MRCK alpha antibody

GTX102598_39609_IHC_19011506_141.jpg
GTX102598_39609_IFA_19011506_197.jpg
GTX102598_39609_WB_19011506_444.jpg

Cat No. GTX102598

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

197 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRCK alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cdc42 Binding Protein Kinase Alpha,Mrck,Mrcka,Pk428,Cdc42Bpa

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102598_39609_IHC_19011506_141.jpg

GTX102598 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MRCK alpha(GTX102598) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102598_39609_IFA_19011506_197.jpg

GTX102598 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using MRCK alpha(GTX102598) antibody at 1:500 dilution.

GTX102598_39609_WB_19011506_444.jpg

GTX102598 WB Image

MRCK alpha antibody detects CDC42BPA protein by Western blot analysis.
A. 30 µg U87-MG whole cell lysate/extract
5 % SDS-PAGE
MRCK alpha antibody (GTX102598) dilution: 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169