APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG
Predict Reactivity
Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 40% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MRCK alpha. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_1240609
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CDC42 binding protein kinase alpha , MRCK , MRCKA , PK428
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX102598 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MRCK alpha(GTX102598) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102598 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HCT116, using MRCK alpha(GTX102598) antibody at 1:500 dilution.
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GTX102598 WB Image
MRCK alpha antibody detects CDC42BPA protein by Western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
5 % SDS-PAGE
MRCK alpha antibody (GTX102598) dilution: 1:1000
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REFERENCE
There are currently no references for MRCK alpha antibody (GTX102598). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MRCK alpha antibody (GTX102598). Be the first to share your experience with this product.
