MRGPRF antibody [HL4802]

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MRGPRF antibody [HL4802] (GTX644814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Unboiled A375 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MRGPRF antibody [HL4802] (GTX644814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MRGPRF antibody [HL4802] (GTX644814) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.