GeneTex
United States (US)

MRLC2 antibody

Comparison
GTX102091_42319_20161110_WB_competitor_watermark_19011506_887.jpg
GTX102091_42319_20151210_WB_19011506_677.jpg

Cat No. GTX102091

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

K562 , Jurkat , Raji , NCI-H929

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRLC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myosin Light Chain 12B,Mlc-B,Mrlc2,Myl12B

Background

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM]

Database

DATA IMAGES
GTX102091_42319_20161110_WB_competitor_watermark_19011506_887.jpg

GTX102091 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with MRLC2 antibody (GTX102091) diluted at 1:5000 and competitor's antibody (SC-15370) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX102091_42319_20151210_WB_19011506_677.jpg

GTX102091 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with MRLC2 antibody (GTX102091) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169