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MRP1 antibody [N1N2], N-term

Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046
Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046
Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046
Anti-MRP1 antibody [N1N2], N-term used in Western Blot (WB). GTX116046
Anti-MRP1 antibody [N1N2], N-term used in Western Blot (WB). GTX116046

Cat. No. GTX116046

Reference 16
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MRP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

172 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human MRP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036140

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily C member 1 , ABC29 , ABCC , GS-X , MRP , MRP1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternative splicing by exon deletion results in several splice variants but maintains the original open reading frame in all forms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046

GTX116046 IHC-P Image

MRP1 antibody [N1N2], N-term detects MRP1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
MRP1 stained by MRP1 antibody [N1N2], N-term (GTX116046) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046

GTX116046 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using MRP1(GTX116046) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046

GTX116046 IHC-P Image

MRP1 antibody [N1N2], N-term detects MRP1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
MRP1 stained by MRP1 antibody [N1N2], N-term (GTX116046) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MRP1 antibody [N1N2], N-term used in Western Blot (WB). GTX116046

GTX116046 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MRP1 antibody [N1N2], N-term (GTX116046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MRP1 antibody [N1N2], N-term used in Western Blot (WB). GTX116046

GTX116046 WB Image

The data was published in the journal Sci Rep in 2018. PMID: 30310131

REFERENCE

Application Reference

Cheng FE et al. Aging (Albany NY) 2024; 16 (4) : 3386-3403 Investigation on the mechanism of the combination of eremias multiocellata and cisplatin in reducing chemoresistance of gastric cancer based on in vitro and in vivo experiments.
Onuma S et al. Cells 2021; 10 (5) Upregulation of Chemoresistance by Mg2+ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.
Application : WB
Reactivity : Human
Wang SA et al. Cell Death Differ 2021; (Epub) USP24 promotes drug resistance during cancer therapy.
Application : WB
Reactivity : Human
Lanzillotta C et al. Prog Neurobiol 2020; : 101892 Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention.
Application : WB
Reactivity : Human
Maruhashi R et al. Sci Rep 2019; 9 (1) : 13753 Chrysin enhances anticancer drug-induced toxicity mediated by the reduction of claudin-1 and 11 expression in a spheroid culture model of lung squamous cell carcinoma cells.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

MRP1 antibody [N1N2], N-term Cat. No. GTX116046

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-MRP1 antibody [N1N2], N-term used in Western Blot (WB). GTX116046
Date : Anonymous submitted on 28-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : H460 cells
Amount used : 15μg
Blocking : Blocking buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Figure Note : 6% SDS-PAGE
Anti-MRP1 antibody [N1N2], N-term used in Western Blot (WB). GTX116046
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: Doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line
Submit a Review
Application Reference
Cheng FE et al. Aging (Albany NY) 2024; 16 (4):3386-3403 Investigation on the mechanism of the combination of eremias multiocellata and cisplatin in reducing chemoresistance of gastric cancer based on in vitro and in vivo experiments.
Onuma S et al. Cells 2021; 10 (5) Upregulation of Chemoresistance by Mg2+ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.
Application : WB
Reactivity : Human
Wang SA et al. Cell Death Differ 2021; (Epub) USP24 promotes drug resistance during cancer therapy.
Application : WB
Reactivity : Human
Lanzillotta C et al. Prog Neurobiol 2020;:101892 Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention.
Application : WB
Reactivity : Human
Maruhashi R et al. Sci Rep 2019; 9 (1):13753 Chrysin enhances anticancer drug-induced toxicity mediated by the reduction of claudin-1 and 11 expression in a spheroid culture model of lung squamous cell carcinoma cells.
Application : WB
Reactivity : Human
Liu L et al. Mol Cancer Ther 2019; (Epub)
Eguchi H et al. Sci Rep 2018; 8 (1):15157 Increase in resistance to anticancer drugs involves occludin in spheroid culture model of lung adenocarcinoma A549 cells.
Application : WB
Reactivity : Human
Liang X et al. Br J Cancer 2017; 116 (7):903-911 Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells.
Meng Q et al. Oncol Lett 2017; 14 (3):3089-3095 MEK inhibitor enhances sensitivity to chemotherapeutic drugs in multidrug resistant hepatocellular carcinoma cells.
Maruhashi R et al. Biochim Biophys Acta 2017; 1865 (3):470-479 Elevation of sensitivity to anticancer agents of human lung adenocarcinoma A549 cells by knockdown of claudin-2 expression in monolayer and spheroid culture models.
Application : WB
Reactivity : Human
Sun SP et al. Nano Futures 2017; (Epub) A Co-Delivery Nanosystem of Chemotherapeutics and DNAzyme Overcomes Cancer Drug Resistance and Metastasis.
Application : WB
Reactivity : Human
Lin GL et al. PLoS One 2017; 12 (10):e0185625 modulation of the mrna-binding protein hur as a novel reversal mechanism of *-triggered multidrug resistance in colorectal cancer cells.
Application : WB
Reactivity : Human
Shengchao Lin et al. The International Journal of Biochemistry & Cell Biology 2015; enhancement of * sensitivity in human colorectal cancer by hypericin mediated photodynamic therapy via ros-related mechanism.
Application : WB
Reactivity : Human
Jiangxia Cai et al. Phytomedicine 2014; 21 (12):1725–1732 salvianolic acid a reverses * resistance in human breast cancer mcf-7 cells via targeting the expression of transgelin 2 and attenuating pi3 k/akt pathway
Application : WB
Reactivity : Human
Cai J et al. Phytomedicine 2014; paeonol reverses * resistance in human breast cancer cells by regulating the expression of transgelin 2.
Application : WB
Reactivity : Human
Chen S et al. Mol Biosyst 2014; 10 (2):294-303 proteomic analysis of the proteins that are associated with the resistance to * in human breast cancer cells.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169