GeneTex
  • Country / Location Selection

United States (US)

MRP1 antibody [N1N2], N-term

Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046
Anti-MRP1 antibody [N1N2], N-term used in Western Blot (WB). GTX116046
Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046
Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046
Anti-MRP1 antibody [N1N2], N-term used in Western Blot (WB). GTX116046

Cat. No. GTX116046

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

172 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human MRP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036140

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily C member 1 , ABC29 , ABCC , GS-X , MRP , MRP1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternative splicing by exon deletion results in several splice variants but maintains the original open reading frame in all forms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046

GTX116046 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using MRP1(GTX116046) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MRP1 antibody [N1N2], N-term used in Western Blot (WB). GTX116046

GTX116046 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MRP1 antibody [N1N2], N-term (GTX116046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046

GTX116046 IHC-P Image

MRP1 antibody [N1N2], N-term detects MRP1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
MRP1 stained by MRP1 antibody [N1N2], N-term (GTX116046) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MRP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116046

GTX116046 IHC-P Image

MRP1 antibody [N1N2], N-term detects MRP1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
MRP1 stained by MRP1 antibody [N1N2], N-term (GTX116046) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MRP1 antibody [N1N2], N-term used in Western Blot (WB). GTX116046

GTX116046 WB Image

The data was published in the journal Sci Rep in 2018. PMID: 30310131

REFERENCE

REVIEW

MRP1 antibody [N1N2], N-term Cat. No. GTX116046

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 28-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : H460 cells
Amount used : 15μg
Blocking : Blocking buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Figure Note : 6% SDS-PAGE
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: Doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169