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MRP4 antibody [M4I-10]

Overexpression
Anti-MRP4 antibody [M4I-10] used in Western Blot (WB). GTX15602

Cat. No. GTX15602

Reference 7

Host

 Rat

Clonality

 Monoclonal

Clone Name

 M4I-10

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, FACS

Reactivity

 Human, Mouse
Package
500 μl ($499)
See all MRP4 products

APPLICATION

Application Note

FACS: Use at a dilution of 1/20 - 1/50. ICC: Use at a dilution of 1/20 - 1/50. IHC-Fr (acetone fixed): Use at a dilution of 1/20. WB: Use at an assay dependent dilution. Predicted molecular weight: 159 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

150 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant, 0.7% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the human MRP4 protein corresponding to amino acids 372-431.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_367212

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily C member 4 , MOAT-B , MOATB , MRP4

Cellular Localization

Membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES

Anti-MRP4 antibody [M4I-10] used in Western Blot (WB). GTX15602

GTX15602 WB Image

Overexpression

The data was published in the journal Int J Mol Sci in 2017. PMID: 28677646

REFERENCE

Application Reference

Mariscal J et al. J Extracell Vesicles 2020; 9 (1) : 1764192 Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.
Javier Mariscal et al. bioRxiv 2019; (Epub) Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.
Application : FACS
Reactivity : Human
Tsukamoto M et al. Cells 2019; 8 (1) A Human ABC Transporter ABCC4 Gene SNP (rs11568658, 559 G > T, G187W) Reduces ABCC4-Dependent Drug Resistance.
Application : WB
Reactivity : Human
Tsukamoto M et al. Int J Mol Sci 2017; 18 (7) Quantitative Evaluation of Drug Resistance Profile of Cells Expressing Wild-Type or Genetic Polymorphic Variants of the Human ABC Transporter ABCC4.
Application : WB
Reactivity : Human
Hazen M et al. MAbs 2014; 6 (1) : 95-107 An improved and robust DNA immunization method to develop antibodies against extracellular loops of multi-transmembrane proteins.
Application : WB
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 499