APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1.5μg/ml |
| 4-6μg/ml |
|
Note :
IHC-P
Human Kidney shows membranous staining in DCT. Paraffin embedded Human Liver.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88691-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_005679.2; NP_001018881.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence KDIDIGKEYIIP-C, from the N Terminus of the protein sequence according to NP_005679.2; NP_001018881.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATP binding cassette subfamily C member 5 , ABC33 , EST277145 , MOAT-C , MOATC , MRP5 , SMRP , pABC11
Cellular Localization
Membrane; Multi-pass membrane protein
Background
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
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GTX88691 WB Image
WB analysis of human frontal cortex lysate using GTX88691 MRP5 antibody, N-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88691 IHC-P Image
IHC-P analysis of human liver using GTX88691 MRP5 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
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GTX88691 IHC-P Image
IHC-P analysis of human kidney using GTX88691 MRP5 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
|
REFERENCE
There are currently no references for MRP5 antibody, N-term (GTX88691). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MRP5 antibody, N-term (GTX88691). Be the first to share your experience with this product.
