Western blotting: use at a dilution of 1/20 to 1/50. FACS: assay dependent. Immunocytochemistry: use at a dilution of 1/20 - 1/50 on acetone fixed cell preparations. Immunohistochemistry: use at a dilution of 1/20 - 1/50 on frozen and formaldehyde-fixed paraffin-embedded tissuesections. Optimal dilutions / concentrations should be determined by the end user. For optimal staining in IHC, the sections should be pretreated with an antigen unmaskingsolution, for example a sodium citrate based buffer. The primary antibodyshould be incubated for about 60 minutes at room temperature when performing IHC.
Liver, kidney and cell Lines (drug-sensitive parental cell lines and their multidrug-resistant derivatives).
This antibody detects with MRP 6. This antibody does not cross-react with MDR 1,MRP 1, MRP 2 or MRP 3, MRP 4 & MRP 5.
Tissue culture supernatant with 0.1% sodium azide and 1% BSA
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Batch dependent (Please refer to the vial label for the specific concentration.)
Bacterial fusion protein of human MRP 6, containing amino acids 764-964, spanning the putative 12th transmembrane region as well as predicted internal and external regions of the protein.
Tissue culture supernatant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ATP binding cassette subfamily C member 6 , ABC34 , ARA , EST349056 , GACI2 , MLP1 , MOAT-E , MOATE , MRP6 , PXE , PXE1 , URG7
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). The encoded protein, a member of the MRP subfamily, is involved in multi-drug resistance. Mutations in this gene cause pseudoxanthoma elasticum. Alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]
GTX23381 IHC Image
MRP 6 Immunohistochemistry