APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , Raji
Predict Reactivity
Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MRPL10. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10722803
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
mitochondrial ribosomal protein L10 , L10MT , MRP-L10 , MRP-L8 , MRPL8 , RPML8
Cellular Localization
Mitochondrion
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-MRPL10 antibody [N2C3] used in Western Blot (WB). GTX118663](/templates/default/js/lightbox/images/loading.gif "Anti-MRPL10 antibody [N2C3] used in Western Blot (WB). GTX118663")
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GTX118663 WB Image
Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX118663 diluted at 1:500
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GTX118663 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human hepatoma, using MRPL10(GTX118663) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for MRPL10 antibody [N2C3] (GTX118663). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MRPL10 antibody [N2C3] (GTX118663). Be the first to share your experience with this product.
![Anti-MRPL10 antibody [N2C3] used in Western Blot (WB). GTX118663](/upload/website/prouct_img/small/GTX118663/GTX118663_40408_WB_18121410_437.jpg "Anti-MRPL10 antibody [N2C3] used in Western Blot (WB). GTX118663")
![Anti-MRPL10 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX118663](/upload/website/prouct_img/small/GTX118663/GTX118663_40408_IHC_18121410_568.jpg "Anti-MRPL10 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX118663")
![Anti-MRPL10 antibody [N2C3] used in Western Blot (WB). GTX118663](/upload/website/prouct_img/normal/GTX118663/GTX118663_40408_WB_18121410_437.jpg "Anti-MRPL10 antibody [N2C3] used in Western Blot (WB). GTX118663")
![Anti-MRPL10 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX118663](/upload/website/prouct_img/normal/GTX118663/GTX118663_40408_IHC_18121410_568.jpg "Anti-MRPL10 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX118663")