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MRPL11 antibody [N1C3]

Anti-MRPL11 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX118773
Anti-MRPL11 antibody [N1C3] used in Western Blot (WB). GTX118773
Anti-MRPL11 antibody [N1C3] used in Western Blot (WB). GTX118773

Cat. No. GTX118773

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all MRPL11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPL11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172990

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein L11 , CGI-113 , L11MT , MRP-L11

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode different isoforms. Pseudogenes corresponding to this gene are found on chromosomes 5q and 12q. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MRPL11 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX118773

GTX118773 IHC-P Image

MRPL11 antibody [N1C3] detects MRPL11 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded lung carcinoma.
MRPL11 antibody [N1C3] (GTX118773) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MRPL11 antibody [N1C3] used in Western Blot (WB). GTX118773

GTX118773 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MRPL11 antibody [N1C3] (GTX118773) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MRPL11 antibody [N1C3] used in Western Blot (WB). GTX118773

GTX118773 WB Image

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
GTX118773 diluted at 1:1000

REFERENCE

Application Reference

Pulman J et al. Hum Mol Genet 2018; (Epub) Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.
Application : WB
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169