GeneTex
United States (US)

MRPL12 antibody [N1C3]

GTX114731_WB_18121410_719.jpg
GTX114731_40233_IHC_18121410_867.jpg
GTX114731_40233_IFA_18121410_651.jpg
GTX114731_40233_WB_M_brain_18121410_571.jpg

Cat No. GTX114731

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , mouse brain

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPL12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitochondrial Ribosomal Protein L12,5C5-2,L12Mt,Mrp-L31/34,Mrpl7,Mrpl7/L12,Rpml12,Mrpl12

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114731_WB_18121410_719.jpg

GTX114731 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX114731 diluted at 1:1000

GTX114731_40233_IHC_18121410_867.jpg

GTX114731 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MRPL12(GTX114731) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114731_40233_IFA_18121410_651.jpg

GTX114731 ICC/IF Image

MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: MRPL12 protein stained by MRPL12 antibody [N1C3] (GTX114731) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX114731_40233_WB_M_brain_18121410_571.jpg

GTX114731 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114731 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169