GeneTex
United States (US)

MRPL13 antibody

MRPL13-antibody-GTX87614-IHC-1_18122102_319.jpg
MRPL13-antibody-GTX87614-WB-1_18122102_314.jpg

Cat No. GTX87614

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

20 kDa. ( Note )

Specificity/Sensitivity

MRPL13 Antibody detects endogenous levels of total MRPL13 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPL13.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitochondrial Ribosomal Protein L13,L13,L13A,L13Mt,Rpl13,Rpml13,Mrpl13

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
MRPL13-antibody-GTX87614-IHC-1_18122102_319.jpg

GTX87614 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MRPL13 Antibody. The picture on the right is treated with the synthesized peptide.

MRPL13-antibody-GTX87614-WB-1_18122102_314.jpg

GTX87614 WB Image

Western blot analysis of extracts from A549 cells, using MRPL13 Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339