GeneTex
United States (US)

MRPL14 antibody

GTX123143_40695_IHC_18121410_102.jpg
GTX123143_40695_WB_18121410_533.jpg
GTX123143_40695_IHC_R_18121410_665.jpg

Cat No. GTX123143

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human MRPL14 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitochondrial Ribosomal Protein L14,L14Mt,L32Mt,Mrp-L14,Mrp-L32,Mrpl32,Rmpl32,Rpml32,Mrpl14

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 17p13.3. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX123143_40695_IHC_18121410_102.jpg

GTX123143 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using MRPL14(GTX123143) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX123143_40695_WB_18121410_533.jpg

GTX123143 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
15% SDS PAGE
GTX123143 diluted at 1:500

GTX123143_40695_IHC_R_18121410_665.jpg

GTX123143 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using MRPL14(GTX123143) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169