GeneTex
United States (US)

MRPL28 antibody [N2C3]

GTX115439_40442_WB_18121410_918.jpg
GTX115439_40442_IHC_18121410_979.jpg

Cat No. GTX115439

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.7mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPL28. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MAAT1 antibody, MGC8499 antibody, p15 antibody, MRPL28 antibody, L28mt antibody, melanoma antigen p15 antibody, melanoma-associated antigen recognised by cytotoxic T lymphocytes antibody, melanoma-associated antigen recognized by T lymphocytes antibody, "39S ribosomal protein L28, mitochondrial antibody", MRP-L28 antibody, mitochondrial ribosomal protein L28 antibody

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX115439_40442_WB_18121410_918.jpg

GTX115439 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX115439 diluted at 1:3000

GTX115439_40442_IHC_18121410_979.jpg

GTX115439 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MRPL28(GTX115439) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf