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MRPL28 antibody [N2C3]

Anti-MRPL28 antibody [N2C3] used in Western Blot (WB). GTX115439
Anti-MRPL28 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115439

Cat No. GTX115439

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPL28. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724804

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitochondrial ribosomal protein L28 , MAAT1 , p15

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MRPL28 antibody [N2C3] used in Western Blot (WB). GTX115439

GTX115439 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX115439 diluted at 1:3000

Anti-MRPL28 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115439

GTX115439 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MRPL28(GTX115439) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for MRPL28 antibody [N2C3] (GTX115439). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for MRPL28 antibody [N2C3] (GTX115439). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169