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MRPL28 antibody

Anti-MRPL28 antibody used in Western Blot (WB). GTX54321
Anti-MRPL28 antibody used in IHC (Paraffin sections) (IHC-P). GTX54321
Anti-MRPL28 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54321
Anti-MRPL28 antibody used in Immunoprecipitation (IP). GTX54321
Anti-MRPL28 antibody used in IHC (Paraffin sections) (IHC-P). GTX54321
Anti-MRPL28 antibody used in IHC (Paraffin sections) (IHC-P). GTX54321

Cat. No. GTX54321

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399)
See all MRPL28 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein L28 , MAAT1 , p15

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MRPL28 antibody used in Western Blot (WB). GTX54321

GTX54321 WB Image

WB analysis of various sample lysates using GTX54321 MRPL28 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-MRPL28 antibody used in IHC (Paraffin sections) (IHC-P). GTX54321

GTX54321 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX54321 MRPL28 antibody.
Dilution : 1:100

Anti-MRPL28 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54321

GTX54321 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54321 MRPL28 antibody.
Blue : DAPI

Anti-MRPL28 antibody used in Immunoprecipitation (IP). GTX54321

GTX54321 IP Image

IP analysis of 293T cell lysate using GTX54321 MRPL28 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-MRPL28 antibody used in IHC (Paraffin sections) (IHC-P). GTX54321

GTX54321 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX54321 MRPL28 antibody.
Dilution : 1:100

Anti-MRPL28 antibody used in IHC (Paraffin sections) (IHC-P). GTX54321

GTX54321 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX54321 MRPL28 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399