APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:100 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
mitochondrial ribosomal protein L28 , MAAT1 , p15
Cellular Localization
Mitochondrion
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX54321 IHC-P Image
IHC-P analysis of rat ovary tissue using GTX54321 MRPL28 antibody.
Dilution : 1:100
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GTX54321 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX54321 MRPL28 antibody.
Dilution : 1:100
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GTX54321 WB Image
WB analysis of various sample lysates using GTX54321 MRPL28 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX54321 IP Image
IP analysis of 293T cell lysate using GTX54321 MRPL28 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000
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GTX54321 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX54321 MRPL28 antibody.
Dilution : 1:100
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GTX54321 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX54321 MRPL28 antibody.
Blue : DAPI
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REFERENCE
There are currently no references for MRPL28 antibody (GTX54321). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MRPL28 antibody (GTX54321). Be the first to share your experience with this product.
