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MRPL32 antibody

Anti-MRPL32 antibody used in Western Blot (WB). GTX87619
Anti-MRPL32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87619

Cat. No. GTX87619

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($409)
See all MRPL32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPL32 (101-150).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HSPC283 , L32mt , MRPL32 , bMRP59b , mitochondrial ribosomal protein L32

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MRPL32 antibody used in Western Blot (WB). GTX87619

GTX87619 WB Image

WB analysis of MCF-7, HepG2, and Jurkat cell lysates using GTX87619 MRPL32 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-MRPL32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87619

GTX87619 ICC/IF Image

ICC/IF analysis of A549 cells using GTX87619 MRPL32 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409