GeneTex
United States (US)

MRPL32 antibody

MRPL32-antibody-GTX87619-ICC_IF-1_18122102_529.jpg
MRPL32-antibody-GTX87619-WB-1_18122102_711.jpg

Cat No. GTX87619

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Specificity/Sensitivity

MRPL32 Antibody detects endogenous levels of total MRPL32 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPL32.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MRP-L32, L32mt, mitochondrial, MRPL32, 39S ribosomal protein L32, MRP L32

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
MRPL32-antibody-GTX87619-ICC_IF-1_18122102_529.jpg

GTX87619 ICC/IF Image

Immunofluorescence analysis of A549 cells, using MRPL32 Antibody. The picture on the right is treated with the synthesized peptide.

MRPL32-antibody-GTX87619-WB-1_18122102_711.jpg

GTX87619 WB Image

Western blot analysis of extracts from MCF-7/HepG2/Jurkat cells, using MRPL32 Antibody. The lane on the right is treated with the synthesized peptide.