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MRPL35 antibody

Anti-MRPL35 antibody used in Western Blot (WB). GTX34086
Anti-MRPL35 antibody used in Western Blot (WB). GTX34086

Cat No. GTX34086

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Specificity/Sensitivity

MRP-L35 Polyclonal Antibody detects endogenous levels of MRP-L35 protein.

PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide derived from the Internal region of human MRP-L35 at AA range: 80-160.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Mitochondrial Ribosomal Protein L35 , L35Mt , Mrp-L35 , Mrpl35

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MRPL35 antibody used in Western Blot (WB). GTX34086

GTX34086 WB Image

Western Blot (WB) analysis of 293 using MRP-L35 antibody.

Anti-MRPL35 antibody used in Western Blot (WB). GTX34086

GTX34086 WB Image

Western Blot (WB) analysis of 293T COLO205 using MRP-L35 antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 319