APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , H1299 , HCT-116
Predict Reactivity
Mouse, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MRPL40. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885448
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
mitochondrial ribosomal protein L40 , L40mt , MRP-L22 , MRP-L40 , MRPL22 , NLVCF , URIM
Cellular Localization
Mitochondrion
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Deletions in this gene may contribute to the etiology of velo-cardio-facial syndrome and DiGeorge syndrome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX119564 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MRPL40 antibody (GTX119564) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX119564 WB Image
MRPL40 antibody detects MRPL40 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MRPL40 antibody (GTX119564) diluted by 1:1000.
|
REFERENCE
There are currently no references for MRPL40 antibody (GTX119564). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MRPL40 antibody (GTX119564). Be the first to share your experience with this product.
