APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/300 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human MRPL42 (AA: 142-203) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitochondrial ribosomal protein L42 , HSPC204 , L31MT , L42MT , MRP-L31 , MRP-L42 , MRP-S32 , MRPL31 , MRPS32 , PTD007 , RPML31 , S32MT
Cellular Localization
Mitochondrion
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq, May 2011]
Database
Research Area
DATA IMAGES
![Anti-MRPL42 antibody [3H6G11] used in Western Blot (WB). GTX60735](/templates/default/js/lightbox/images/loading.gif "Anti-MRPL42 antibody [3H6G11] used in Western Blot (WB). GTX60735")
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GTX60735 WB Image
WB analysis of HL7702 (1), HepG2 (2), SMMC-7721 (3), HEK293 (4), HeLa (5), and Raji (6) cell lysate using GTX60735 MRPL42 antibody [3H6G11].
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GTX60735 IHC-P Image
IHC-P analysis of cervical cancer tissue using GTX60735 MRPL42 antibody [3H6G11].
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GTX60735 ELISA Image
ELISA analysis of antigen using GTX60735 MRPL42 antibody [3H6G11].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60735 IHC-P Image
IHC-P analysis of esophageal cancer tissue using GTX60735 MRPL42 antibody [3H6G11].
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GTX60735 FACS Image
FACS analysis of HepG2 cells using GTX60735 MRPL42 antibody [3H6G11].
Green : MRPL42
Purple : negative control
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GTX60735 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60735 MRPL42 antibody [3H6G11].
Green : MRPL42
Blue: DRAQ5 fluorescent DNA dye
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GTX60735 WB Image
WB analysis of HEK293 (1) and MRPL42 (AA: 142-203)-hIgGFc transfected HEK293 (2) cell lysate using GTX60735 MRPL42 antibody [3H6G11].
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REFERENCE
There are currently no references for MRPL42 antibody [3H6G11] (GTX60735). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MRPL42 antibody [3H6G11] (GTX60735). Be the first to share your experience with this product.
![Anti-MRPL42 antibody [3H6G11] used in Western Blot (WB). GTX60735](/upload/website/prouct_img/small/GTX60735/GTX60735_20170912_WB_1_w_23061123_594.webp "Anti-MRPL42 antibody [3H6G11] used in Western Blot (WB). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in IHC (Paraffin sections) (IHC-P). GTX60735](/upload/website/prouct_img/small/GTX60735/GTX60735_20170912_IHC-P_w_23061123_479.webp "Anti-MRPL42 antibody [3H6G11] used in IHC (Paraffin sections) (IHC-P). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in ELISA (ELISA). GTX60735](/upload/website/prouct_img/small/GTX60735/GTX60735_20170912_ELISA_w_23061123_831.webp "Anti-MRPL42 antibody [3H6G11] used in ELISA (ELISA). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in IHC (Paraffin sections) (IHC-P). GTX60735](/upload/website/prouct_img/small/GTX60735/GTX60735_20170912_IHC-P_1_w_23061123_752.webp "Anti-MRPL42 antibody [3H6G11] used in IHC (Paraffin sections) (IHC-P). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in Flow cytometry (FACS). GTX60735](/upload/website/prouct_img/small/GTX60735/GTX60735_20170912_FACS_w_23061123_221.webp "Anti-MRPL42 antibody [3H6G11] used in Flow cytometry (FACS). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60735](/upload/website/prouct_img/small/GTX60735/GTX60735_20170912_ICCIF_w_23061123_588.webp "Anti-MRPL42 antibody [3H6G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in Western Blot (WB). GTX60735](/upload/website/prouct_img/small/GTX60735/GTX60735_20170912_WB_w_23061123_613.webp "Anti-MRPL42 antibody [3H6G11] used in Western Blot (WB). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in Western Blot (WB). GTX60735](/upload/website/prouct_img/normal/GTX60735/GTX60735_20170912_WB_1_w_23061123_594.webp "Anti-MRPL42 antibody [3H6G11] used in Western Blot (WB). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in IHC (Paraffin sections) (IHC-P). GTX60735](/upload/website/prouct_img/normal/GTX60735/GTX60735_20170912_IHC-P_w_23061123_479.webp "Anti-MRPL42 antibody [3H6G11] used in IHC (Paraffin sections) (IHC-P). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in ELISA (ELISA). GTX60735](/upload/website/prouct_img/normal/GTX60735/GTX60735_20170912_ELISA_w_23061123_831.webp "Anti-MRPL42 antibody [3H6G11] used in ELISA (ELISA). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in IHC (Paraffin sections) (IHC-P). GTX60735](/upload/website/prouct_img/normal/GTX60735/GTX60735_20170912_IHC-P_1_w_23061123_752.webp "Anti-MRPL42 antibody [3H6G11] used in IHC (Paraffin sections) (IHC-P). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in Flow cytometry (FACS). GTX60735](/upload/website/prouct_img/normal/GTX60735/GTX60735_20170912_FACS_w_23061123_221.webp "Anti-MRPL42 antibody [3H6G11] used in Flow cytometry (FACS). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60735](/upload/website/prouct_img/normal/GTX60735/GTX60735_20170912_ICCIF_w_23061123_588.webp "Anti-MRPL42 antibody [3H6G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60735")
![Anti-MRPL42 antibody [3H6G11] used in Western Blot (WB). GTX60735](/upload/website/prouct_img/normal/GTX60735/GTX60735_20170912_WB_w_23061123_613.webp "Anti-MRPL42 antibody [3H6G11] used in Western Blot (WB). GTX60735")