GeneTex
United States (US)

MRPL42 antibody

MRPS32-antibody-GTX87942-IHC-1_18122102_654.jpg

Cat No. GTX87942

Host Rabbit
Clonality Polyclonal
Application IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Specificity/Sensitivity

MRPS32 Antibody detects endogenous levels of total MRPL42 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 210% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPL42.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MRPS32, Mitochondrial 28S ribosomal protein S32, S32mt, MRP-S32, mitochondrial, Mitochondrial28S ribosomal protein S32, MRP S32

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Further experiments will be needed to identify the specific subunit localization. Sequence analysis identified three transcript variants that encode two different isoforms. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
MRPS32-antibody-GTX87942-IHC-1_18122102_654.jpg

GTX87942 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human placenta, using MRPS32 Antibody. The picture on the right is treated with the synthesized peptide.