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MRPL45 antibody

Anti-MRPL45 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66181
Anti-MRPL45 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66181
Anti-MRPL45 antibody used in IHC (Paraffin sections) (IHC-P). GTX66181
Anti-MRPL45 antibody used in Western Blot (WB). GTX66181

Cat. No. GTX66181

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MRPL45 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 57-306 of human MRPL45 (NP_115727.5).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein L45 , L45mt , MRP-L45

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-MRPL45 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66181

GTX66181 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66181 MRPL45 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MRPL45 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66181

GTX66181 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX66181 MRPL45 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MRPL45 antibody used in IHC (Paraffin sections) (IHC-P). GTX66181

GTX66181 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX66181 MRPL45 antibody.
Dilution : 1:100

Anti-MRPL45 antibody used in Western Blot (WB). GTX66181

GTX66181 WB Image

WB analysis of various sample lysates using GTX66181 MRPL45 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399