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MRPS10 antibody [N1C3]

Anti-MRPS10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119522
Anti-MRPS10 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119522
Anti-MRPS10 antibody [N1C3] used in Western Blot (WB). GTX119522

Cat. No. GTX119522

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MRPS10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPS10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618239

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein S10 , MRP-S10 , PNAS-122

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S10P family. Pseudogenes corresponding to this gene are found on chromosomes 1q, 3p, and 9p. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MRPS10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119522

GTX119522 IHC-P Image

MRPS10 antibody [N1C3] detects MRPS10 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: MRPS10 antibody [N1C3] (GTX119522) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MRPS10 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119522

GTX119522 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using MRPS10(GTX119522) antibody at 1:200 dilution.

Anti-MRPS10 antibody [N1C3] used in Western Blot (WB). GTX119522

GTX119522 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX119522 diluted at 1:1000

REFERENCE

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REVIEW

There are currently no reviews for MRPS10 antibody [N1C3] (GTX119522). Be the first to share your experience with this product.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169